This section provides some sample data in OME-TIFF format. They include data produced from an acquisition system as well as artificial sample datasets (i.e. designed for developer testing that illustrate some possible data organizations, which should be useful if you are interested in implementing support for OME-TIFF within your software.
All the OME-TIFF sample data discussed below are available from our OME-TIFF sample images resource.
This section lists various examples of single file OME-TIFF. For examples of OME-TIFF dataset distributed across multiple TIFF files, see Multi-file OME-TIFF filesets.
All datasets in the following table were artificially generated with each plane labeled according to its dimensional position for easy testing. Each one consists of a single OME-TIFF file (2016-06 schema version) containing every constituent image plane.
| Sample | Channels | Focal planes | Time points |
|---|---|---|---|
| single-channel.ome.tif | 1 | 1 | 1 |
| multi-channel.ome.tif | 3 | 1 | 1 |
| z-series.ome.tif | 1 | 5 | 1 |
| time-series.ome.tif | 1 | 1 | 7 |
| multi-channel-z-series.ome.tif | 3 | 5 | 1 |
| multi-channel-time-series.ome.tif | 3 | 1 | 7 |
| 4D-series.ome.tif | 1 | 5 | 7 |
| multi-channel-4D-series.ome.tif | 3 | 5 | 7 |
Sample files implementing the 6D, 7D and 8D storage are available from the Modulo folder of the image downloads resource.
This section lists various examples of OME-TIFF datasets distributed across multiple TIFF files.
The following OME-TIFF datasets consist of tubulin histone GFP coexpressing C. elegans embryos. Many thanks to Josh Bembenek for preparing and imaging this sample data.
The datasets were acquired on a multiphoton workstation (2.1 GHz Athlon XP 3200+ with 1GB of RAM) using WiscScan. All image planes were collected at 512x512 resolution in 8-bit grayscale, with an integration value of 2.
The files available for download have been updated to the current schema version since their initial creation.
| Fileset | Zipped fileset | Channels | Focal planes | Time points | Number of files |
|---|---|---|---|---|---|
| tubhiswt-2D | tubhiswt-2D.zip | 2 | 1 | 1 | 2 |
| tubhiswt-3D | tubhiswt-3D.zip | 2 | 1 | 20 | 2 |
| tubhiswt-4D | tubhiswt-4D.zip | 2 | 10 | 43 | 86 |
The master OME-TIFF fileset and companion OME-XML fileset both contain a set of 18 by 24 pixel images with black and white text on each plane giving its time, z-depth and channel. Each of the five focal planes is saved as a separate OME-TIFF named multifile-Zxx.ome.tiff where xx is the index of the focal plane.
For this sample, the full OME-XML metadata describing the whole fileset is embedded into a master OME-TIFF file. Partial OME-XML metadata blocks are embedded into the four other OME-TIFF files and refer to the master OME-TIFF file as described in the specification.
For this sample, the full OME-XML metadata describing the whole fileset is stored into a separate companion OME-XML file. Partial OME-XML metadata blocks are embedded into the five OME-TIFF files and refer to the companion OME-XML file as described in the specification.