OMERO.server installation

This section covers the installation of OMERO.server on UNIX and UNIX-like platforms. This includes all BSD, Linux and Mac OS X systems. Depending upon which platform you are using, you may find a more specific walk-through listed below but we recommend you read through this page first as it explains the entire process rather than just being a series of commands. The walk-throughs describs how to install the recommended versions, not all the supported versions. This should be read in conjunction with Version requirements.

Since 5.6, a new OMERODIR variable is used, you should first unset OMERO_HOME (if set) before beginning the installation process.

Recommended:

OMERO.server installation on CentOS 7

Instructions for installing OMERO.server from scratch on CentOS 7 with Ice 3.6 and Python 3.6.

OMERO.server installation on Debian 10

Instructions for installing OMERO.server from scratch on Debian 10 with Ice 3.6 and Python 3.7.

OMERO.server installation on Ubuntu 18.04

Instructions for installing OMERO.server from scratch on Ubuntu 18.04 with Ice 3.6 and Python 3.6.

Upcoming:

OMERO.server installation on CentOS 8

Instructions for installing OMERO.server from scratch on CentOS 8 with Ice 3.6 and Python 3.6.

OMERO.server installation on Ubuntu 20.04

Instructions for installing OMERO.server from scratch on Ubuntu 20.04 with Ice 3.6 and Python 3.8.

OMERO.server installation on Debian 9

Instructions for installing OMERO.server from scratch on Debian 9 with Ice 3.6 and Python 3.5.

Development:

OMERO.server installation on OS X with Homebrew

Instructions for installing and building OMERO.server on Mac OS X with dependencies installed using Homebrew. It is aimed at developers since typically MacOS X is not suited for serious server deployment.

Prerequisites

Installation will require:

  • a clean, minimal operating system installation

  • a “root” level account for which you know the password

Note

If you are unsure of what it means to have a “root” level account, or if you are generally having issues with the various users/passwords described in this install guide, please see Which user account and password do I use where?.

The installation and configuration of the prerequisite applications are mostly outside the scope of this document. For Linux distributions, use of the default package manager is recommended. For MacOS X, Homebrew is recommended. This guide provides the package names to install for a number of contemporary systems. However, the names and versions provided vary between releases. Please do check for similar packages if the one documented here is not available for your system as it may be provided under an alternative name. “Debian” refers to Debian and derivative distributions such as Ubuntu. “RedHat” refers to RedHat and related distributions such as CentOS, Fedora and Scientific Linux.

  • For Ubuntu you need to enable the universe repository. This should be enabled by default. If not enabled, it may be enabled by editing /etc/apt/sources.list directly, in which case the entries may already exist but are commented out, or by using Synaptic (10.04 and 10.10) or Ubuntu Software Center (11.04 onwards). Update your package lists to ensure that you get the latest packages:

    $ sudo apt-get update
    

    Install packages by running:

    $ sudo apt-get install package
    

    where package is the package name to install.

The following subsections cover the details for each package, in the order recommended for installation.

Java SE Runtime Environment (JRE)

If possible, install one of the following packages:

System

Package

Debian

openjdk-11-jre

Homebrew

N/A (install Oracle Java)

RedHat

java-11-openjdk

OMERO works with the OpenJDK JRE provided by most systems, or with Oracle Java. Version 11 or later is required.

Your system may already provide a suitable JRE, in which case no extra steps are necessary. Linux distributions usually provide OpenJDK, and older MacOS X versions have Java installed by default. Oracle Java is no longer provided by BSD or Linux distributions for licensing reasons. If your system does not have Java available, for example on newer MacOS X versions, or the provided version is too old, Oracle Java may be downloaded from the Oracle website.

Warning

Security

Installing Oracle Java outside the system’s package manager will leave your system without regular distribution-supplied security updates, and so is not recommended.

To check which version of Java is currently available:

$ which java
/usr/bin/java
$ java -version
openjdk version "11.0.5" 2019-10-15
OpenJDK Runtime Environment (build 11.0.5+10-post-Ubuntu-0ubuntu1.118.04)
OpenJDK 64-Bit Server VM (build 11.0.5+10-post-Ubuntu-0ubuntu1.118.04, mixed mode, sharing)

Python 3

Check you have Python (and check its version) by running:

$ python3 --version
Python 3.6.4

If possible, install the following packages:

System

Package

Debian

python3

Homebrew

python3

RedHat

python3

Ice

The Ice version may vary, depending upon the distribution version you are using. The Ice versions in currently supported versions of Debian and Ubuntu are shown in the Ice of the Version requirements page.

Using version 3.6 of Ice is required. If your package manager provides Ice packages, using these is recommended where possible. Distribution-provided packages often have additional bugfixes which are not present in the upstream releases.

If needed, source and binary packages are available from ZeroC. The latest release is available from the ZeroC website.

Note

ZeroC Ice can always be built from source code for specific platforms if a binary package is not available.

Note

With Ice 3.6, the Python bindings are provided separately. If your package manager does not provide Ice python packages, run pip install zeroc-ice to install the Ice Python bindings. See Using the Python Distribution for further details.

OMERO.scripts

If you wish to run the “Movie Maker” script, please install mencoder.

System

Package

Debian

mencoder

Homebrew

mplayer

RedHat

mencoder

Installation

Once the above prerequisites have been downloaded, installed and configured appropriately, the OMERO server itself may be installed. You may wish to create a user account solely for the purpose of running the server, and switch to this user for the next steps.

Server directory

Firstly, a directory needs to be created to contain the server. In this case ~/omero is used as an example:

$ mkdir -p ~/omero

Next, change into this directory:

$ cd ~/omero

OMERO.server

The release OMERO.server.zip is available from the OMERO downloads page. Download the version matching the version of Ice installed on your system before continuing.

Installing a development version from source is also possible. See the Installing OMERO from source section for further details. This is not recommended unless you have a specific reason not to use a release version.

Once you have obtained the OMERO.server zip archive matching the version of Ice installed, unpack it:

$ unzip OMERO.server-5.6.2-ice36-byy.zip

If your system does not provide an unzip command by default, install one of the following:

System

Package

Debian

unzip

Homebrew

unzip

RedHat

unzip

Optionally, give your OMERO software install a short name to save some typing later, to reflect what you set OMERO_PREFIX to in the Environment variables section, below:

$ ln -s OMERO.server-5.6.2-ice36-byy OMERO.server

This will also ease installation of newer versions of the server at a later date, by simply updating the link.

Environment variables

If using distribution-provided packages such as Debian or RPM packages, or via the homebrew or macports package manager, it should not be necessary to set any environment variables. However, if using third-party packages for any required components, several variables may require setting in order for them to function correctly.

Please note that the precise details of these environment variables can change as new versions of software are released.

There are several methods for setting environment variables; which is most appropriate will depend upon how the OMERO server is started. Options include:

/etc/security/pam_env.conf

Global environment set at login by PAM

/etc/profile or /etc/profile.d/omero

Global Bourne shell defaults (also used by derived shells such as bash and zsh)

~/.profile

User’s Bourne shell defaults (also used by derived shells)

/etc/bash.bashrc

Global bash defaults

~/.bashrc, ~/.bash_profile or ~/.bash_login

User’s bash configuration.

If OMERO is started as a service using an init script, a global setting should be preferred. If being started by hand using a particular user, a user-specific configuration file may be more appropriate.

The following environment variables may be configured:

LD_LIBRARY_PATH (Linux) or DYLD_LIBRARY_PATH (MacOS X)

The Ice and PostgreSQL libraries must be on the library search path. If using the packages provided by your distribution, this will already be the case. If using third-party binary distributions the lib (or lib64 if present and using a 64-bit system) directory for each will require adding to the library search path.

OMERO_PREFIX

This is not strictly required, but may be set for convenience to point to the OMERO server installation, and is used in this documentation as a shorthand for the installation path.

OMERO_TMPDIR

Directory used for temporary files. If the home directory of the user running the OMERO server is located on a slow filesystem, such as NFS, this may be used to store the temporary files on fast local storage.

PATH

The search path must include the programs java, python, icegridnode and PostgreSQL commands such as psql. If using the packages provided by your distribution, this will already be the case. If using third-party binary distributions such as the ZeroC Ice package, Oracle Java, or PostgreSQL, the bin directory for each must be added to the path. The OMERO bin directory may also be added to the search path ($OMERO_PREFIX/bin if OMERO_PREFIX has been set).

PYTHONPATH

The Ice python directory must be made available to python. If using the Ice packages provided by your distribution, this will already be the case. If using the ZeroC ice package, add the python directory to the python path. For Ice 3.6, this should never be required.

OMERODIR

The path to the OMERO.server.

After making any needed changes, either source the corresponding file or log back in for them to take effect. Run env to check them.

Creating a database

On most systems, a “postgres” user will be created which has admin privileges, while the UNIX root user itself does not have admin privileges. Therefore it is necessary to either become the postgres user, or use sudo as shown below.

For the purposes of this guide, the following dummy data is used:

Username: db_user
Password: db_password
Database: omero_database

Warning

Security

These dummy values are examples only and should not be used. For a live or public server install these values should be altered to reflect your security requirements—i.e. use your own choice of username and password instead. These should not be the same username and/or password as your Linux/Mac root user!

You should also consider restricting access to your server machine, but that is outside the scope of this document.

  • Create a non-superuser database user and record the name and password used. You will need to configure OMERO to use this username and password later on.:

    $ sudo -u postgres createuser -P -D -R -S db_user
    Enter password for new role:       # db_password
    Enter it again:       # db_password
    
  • Create a database for OMERO to reside in:

    $ sudo -u postgres createdb -E UTF8 -O db_user omero_database
    
  • Check to make sure the database has been created, you have PostgreSQL client authentication correctly set up and the database is owned by the db_user user.

    $ psql -h localhost -U db_user -l
    Password for user db_user:
            List of databases
       Name         |  Owner   | Encoding
    ----------------+----------+-----------
     omero_database | db_user  | UTF8
     postgres       | postgres | UTF8
     template0      | postgres | UTF8
     template1      | postgres | UTF8
    (4 rows)
    

If you have problems, especially with the last step, take a look at OMERO.server and PostgreSQL since the authentication mechanism is probably not properly configured.

Location for the your OMERO binary repository

  • Create a directory for the OMERO binary data repository. /OMERO is the default location and should be used unless you explicitly have a reason not to and know what you are doing.

  • This is not where you want the OMERO application to be installed, it is a separate directory which the OMERO.server will use to store binary data.

  • You can read more about the OMERO binary repository.

    $ sudo mkdir /OMERO
    
  • Change the ownership of the directory. /OMERO must either be owned by the user starting the server (it is currently owned by the system root) or that user must have permission to write to the directory. You can find out your username and edit the correct permissions as follows:

    $ whoami
    omero
    $ sudo chown -R omero /OMERO
    

Configuration

  • You can view a parsed version of the configuration properties under Configuration properties glossary or parse it yourself with omero config parse.

  • Change any settings that are necessary using omero config, including the name and/or password for the ‘db_user’ database user you chose above or the database name if it is not “omero_database”. (Quotes are only necessary if the value could be misinterpreted by the shell. See Forum post)

    $ omero config set omero.db.name 'omero_database'
    $ omero config set omero.db.user 'db_user'
    $ omero config set omero.db.pass 'db_password'
    

    You can also check the values that have been set using:

    $ omero config get
    
  • If you have chosen a non-standard OMERO binary repository location above, be sure to configure the omero.data.dir property. For example, to use /srv/omero:

    $ omero config set omero.data.dir /srv/omero
    
  • Create the OMERO database initialization script. You will need to provide a password for the newly created OMERO root user, either by using the --password argument or by entering it when prompted. Note that this password is for the root user of the OMERO.server, and is not related to the root system user or a PostgreSQL user role.

    $ omero db script --password omero_root_password
    
    Using OMERO5.4 for version
    Using 0 for patch
    Using password from commandline
    Saving to /home/omero/OMERO5.4__0.sql
    

Warning

Security

For illustrative purposes, the default password for the OMERO root user is shown as omero_root_password. However, you should not use this default values for your installation, but use your own choice of password instead. This should not be the same password as your Linux/Mac root user or the database user!

  • Initialize your database with the script.

    $ psql -h localhost -U db_user omero_database < OMERO5.4__0.sql

    At this point you should see some output from PostgreSQL as it installs the schema for new OMERO database.

  • Before starting the OMERO.server, run the OMERO diagnostics script to check that all of the settings are correct, e.g.

    $ omero admin diagnostics
    
  • You can now start the server using:

    $ omero admin start
    Creating var/master
    Initializing var/log
    Creating var/registry
    No descriptor given. Using etc/grid/default.xml
    
  • If multiple users have access to the system running OMERO you should restrict access to the OMERO.server/etc and OMERO.server/var directories, for example by changing the permissions on them:

    $ chmod 700 ~/omero/OMERO.server/etc ~/omero/OMERO.server/var
    

    You should also consider restricting access to the OMERO data repository. The required permissions will depend on whether you are using Advanced import scenarios.

  • Test that you can log in as “root”, either with the OMERO.insight client or on the command-line:

    $ omero login
    Server: [localhost]
    Username: [root]
    Password:         # omero_root_password
    

    You will be prompted for an OMERO username and password. Use the username and password set when running omero db script.

  • If your users are going to be importing many files in one go, for example multiple plates, you should make sure you set the maximum number of open files to a sensible level (i.e. at least 8K for production systems, 16K for bigger machines). See Too many open files for more information.

JVM memory settings

The OMERO server starts a number of Java services. Memory settings for these are calculated on a system-by-system basis. An attempt has been made to have usable settings out of the box, but if you can afford to provide OMERO with more memory, it will certainly improve your overall performance. See Memory configuration on how to tune the JVM.

Enabling movie creation from OMERO

OMERO has a facility to create AVI/MPEG Movies from images. The page OMERO.movie details how to enable it.

Post-installation items

Backup

One of your first steps after putting your OMERO server into production should be deciding on when and how you are going to backup your database and binary data. Please do not omit this step.

Security

It is also now recommended that you read the Server security and firewalls page to get a good idea as to what you need to do to get OMERO clients speaking to your newly installed OMERO.server in accordance with your institution or company’s security policy.

Advanced configuration

Once you have the base server running, you may want to try enabling some of the advanced features such as OMERO.dropbox or LDAP authentication. If you have Flex data, you may want to watch the HCS configuration screencast. See Configuration properties glossary on how to get the most out of your server.

Troubleshooting

My OMERO install doesn’t work! What do I do now? Examine the Troubleshooting OMERO page and if all else fails post a message to our ome-users mailing list discussed on the Community support page. Especially the Server fails to start and Remote clients cannot connect to OMERO installation sections are a good starting point.

OMERO diagnostics

If you want help with your server installation, please include the output of the diagnostics command:

$ omero admin diagnostics

================================================================================
OMERO Diagnostics 5.6.2
================================================================================

Commands:   java -version                  11.0.5    (/usr/bin/java)
Commands:   python -V                      3.6.9     (/opt/omero/server/venv3/bin/python)
Commands:   icegridnode --version          3.6.5     (/usr/bin/icegridnode)
Commands:   icegridadmin --version         3.6.5     (/usr/bin/icegridadmin)
Commands:   psql --version                 11.6      (/usr/bin/psql)
Commands:   openssl version                1.1.111   (/usr/bin/openssl)

Server:     icegridnode                    running
Server:     Blitz-0                        active (pid = 30324, enabled)
Server:     DropBox                        active (pid = 30343, enabled)
Server:     FileServer                     active (pid = 30345, enabled)
Server:     Indexer-0                      active (pid = 30348, enabled)
Server:     MonitorServer                  active (pid = 30351, enabled)
Server:     OMERO.Glacier2                 active (pid = 30353, enabled)
Server:     OMERO.IceStorm                 active (pid = 30376, enabled)
Server:     PixelData-0                    active (pid = 30393, enabled)
Server:     Processor-0                    active (pid = 30394, enabled)
Server:     Tables-0                       inactive (disabled)
Server:     TestDropBox                    inactive (enabled)

OMERO:      SSL port                       4064
OMERO:      TCP port                       4063

Log dir:    /opt/omero/server/OMERO.server/var/log exists

Log files:  Blitz-0.log                    22.8 KB        errors=0    warnings=9
Log files:  DropBox.log                    1.3 KB        errors=0    warnings=1
Log files:  FileServer.log                 114 B
Log files:  Indexer-0.log                  1.3 KB       errors=0    warnings=5
Log files:  MonitorServer.log              882 B
Log files:  PixelData-0.log                1.8 KB        errors=0    warnings=4
Log files:  Processor-0.log                592 B        errors=0    warnings=1
Log files:  Tables-0.log                   841 B
Log files:  TestDropBox.log                n/a
Log files:  master.err                     34.4 KB        errors=2    warnings=0
Log files:  master.out                     empty
Log files:  Total size                     0.06 MB

Environment:OMERODIR=/opt/omero/server/OMERO.server
Environment:OMERO_HOME=(unset)
Environment:OMERO_NODE=(unset)
Environment:OMERO_MASTER=(unset)
Environment:OMERO_TEMPDIR=(unset)
Environment:PATH=/opt/omero/server/venv3/bin:/usr/local/bin:/usr/bin:/bin
Environment:ICE_HOME=(unset)
Environment:LD_LIBRARY_PATH=(unset)
Environment:DYLD_LIBRARY_PATH=(unset)

OMERO SSL port:4064
OMERO TCP port:4063
OMERO data dir: '/OMERO'        Exists? True    Is writable? True
OMERO temp dir: '/home/omero-server/tmp'        Exists? True    Is writable? True   (Size: 0)

JVM settings: Blitz-${index}                -Xmx621m -XX:MaxPermSize=512m -XX:+IgnoreUnrecognizedVMOptions
JVM settings: Indexer-${index}              -Xmx414m -XX:MaxPermSize=512m -XX:+IgnoreUnrecognizedVMOptions
JVM settings: PixelData-${index}            -Xmx621m -XX:MaxPermSize=512m -XX:+IgnoreUnrecognizedVMOptions
JVM settings: Repository-${index}           -Xmx414m -XX:MaxPermSize=512m -XX:+IgnoreUnrecognizedVMOptions

Update notification

Your OMERO.server installation will check for updates each time it is started from the Open Microscopy Environment update server. If you wish to disable this functionality you should do so now as outlined on the OMERO upgrade checks page.