Note
This documentation is for the new OMERO 5.2 version. See the latest OMERO 5.1.x version or the previous versions page to find documentation for the OMERO version you are using if you have not upgraded yet.
This installation walkthrough should be read in conjunction with OMERO.server installation and OMERO.web deployment.
Running OMERO on CentOS 6 has a number of special requirements which deviate from the standard installation instructions. The instructions below will set up Python 2.7 and Ice 3.5 on CentOS 6. We tested the installation with Python 2.7 from IUS and used a virtual environment to install the various dependencies required to install an OMERO.server. It is also possible to use SCL Python (for example walkthrough_centos6_py27.sh) but such solution could have potential side effects.
CentOS 6 provides Python 2.6. However, OMERO.web requires Python 2.7 in order to use Django 1.8. While Django 1.6 may be used with Python 2.6, this version of Django no longer has security support. In consequence, it is necessary to upgrade to Python 2.7 in order to obtain Django security updates, which are required for a production deployment.
The RPM packages provided by ZeroC for CentOS 6 use the Python 2.6 provided by the system. OMERO will need an Ice build which uses Python 2.7. A version of Ice 3.5 built against Python 2.7 installed above is available at http://downloads.openmicroscopy.org/ice/experimental. Ice will be installed into /opt.
The following steps are run as root.
Install Java 1.8, Ice 3.5 and PostgreSQL 9.4:
To install Java 1.8 and other dependencies:
# epel-release will be pulled as a dependency
yum -y install https://centos6.iuscommunity.org/ius-release.rpm
# installed for convenience
yum -y install unzip wget tar
# install Java
yum -y install java-1.8.0-openjdk
# install dependencies
yum -y install \
python27 \
python27-devel \
libjpeg-devel \
libpng-devel \
libtiff-devel \
hdf5-devel \
zlib-devel \
freetype-devel
# install pip and virtualenv using Python 2.6
yum -y install python-pip
pip install --upgrade virtualenv
#if virtualenv is not installed (unlikely)
#yum -y install python27-pip
#pip2.7 install virtualenv
# TODO: this installs a lot of unecessary packages:
yum -y groupinstall "Development Tools"
export PYTHONWARNINGS="ignore:Unverified HTTPS request"
To install Ice 3.5:
curl -o /etc/yum.repos.d/zeroc-ice-el6.repo \
http://download.zeroc.com/Ice/3.5/el6/zeroc-ice-el6.repo
yum -y install db53 db53-utils mcpp
mkdir /tmp/ice-download
cd /tmp/ice-download
wget http://downloads.openmicroscopy.org/ice/experimental/Ice-3.5.1-b1-centos6-iuspy27-x86_64.tar.gz
tar -zxvf /tmp/ice-download/Ice-3.5.1-b1-centos6-iuspy27-x86_64.tar.gz
# Install under /opt
mv Ice-3.5.1-b1-centos6-iuspy27-x86_64 /opt/Ice-3.5.1
# make path to Ice globally accessible
# if globally set, there is no need to export LD_LIBRARY_PATH
echo /opt/Ice-3.5.1/lib64 > /etc/ld.so.conf.d/ice-x86_64.conf
ldconfig
To install PostgreSQL 9.4:
# install Postgres
# Postgres, reconfigure to allow TCP connections
yum -y install http://yum.postgresql.org/9.4/redhat/rhel-6-x86_64/pgdg-centos94-9.4-2.noarch.rpm
yum -y install postgresql94-server postgresql94
service postgresql-9.4 initdb
sed -i.bak -re 's/^(host.*)ident/\1md5/' /var/lib/pgsql/9.4/data/pg_hba.conf
chkconfig postgresql-9.4 on
service postgresql-9.4 start
The remaining dependencies will be installed in a virtual environment:
# Install the OMERO dependencies in a virtual environment
# Create virtual env.
# -p only required if virtualenv has been installed with python 2.6
virtualenv -p /usr/bin/python2.7 /home/omero/omeroenv
set +u
source /home/omero/omeroenv/bin/activate
set -u
/home/omero/omeroenv/bin/pip install --upgrade pip
/home/omero/omeroenv/bin/pip2.7 install -r requirements_centos6_py27_ius.txt
deactivate
See requirements_centos6_py27_ius.txt
Create an omero system user, and a directory for the OMERO repository:
useradd -m omero
mkdir -p "$OMERO_DATA_DIR"
chown omero "$OMERO_DATA_DIR"
Create a database user and initialize a new database for OMERO:
echo "CREATE USER $OMERO_DB_USER PASSWORD '$OMERO_DB_PASS'" | \
su - postgres -c psql
su - postgres -c "createdb -E UTF8 -O '$OMERO_DB_USER' '$OMERO_DB_NAME'"
psql -P pager=off -h localhost -U "$OMERO_DB_USER" -l
The following settings will need adding to your OMERO startup script or to the omero user’s environment (for example in a shell startup script). Add the absolute path to the bin directory of the virtual environment /home/omero/omeroenv to the PATH variable:
# Environment file for OMERO
export ICE_HOME=/opt/Ice-3.5.1
export PATH="$ICE_HOME/bin:/home/omero/omeroenv/bin:$PATH"
#Remove commented out export below if Ice is not set globally accessible
#export LD_LIBRARY_PATH="$ICE_HOME/lib64:$ICE_HOME/lib:$LD_LIBRARY_PATH"
export PYTHONPATH="$ICE_HOME/python:$PYTHONPATH"
export SLICEPATH="$ICE_HOME/slice"
These settings will enable Python 2.7, and set the necessary environment variables for Ice 3.5 to work.
For example, download omero-centos6py27ius.env into /home/omero and run:
echo source \~omero/omero-centos6py27ius.env >> ~omero/.bashrc
The following steps are run as the omero system user.
Download, unzip and configure OMERO. The rest of this walkthrough assumes the OMERO.server is installed into the home directory of the omero system user.
Note that this script requires the same environment variables that were set earlier in settings.env, so you may need to copy and/or source this file as the omero user.
You will need to install the server corresponding to your Ice version.
Install server-ice35.zip:
cd ~omero
SERVER=http://downloads.openmicroscopy.org/latest/omero5.2/server-ice35.zip
wget $SERVER
unzip -q OMERO.server*
Configure:
ln -s OMERO.server-*/ OMERO.server
OMERO.server/bin/omero config set omero.data.dir "$OMERO_DATA_DIR"
OMERO.server/bin/omero config set omero.db.name "$OMERO_DB_NAME"
OMERO.server/bin/omero config set omero.db.user "$OMERO_DB_USER"
OMERO.server/bin/omero config set omero.db.pass "$OMERO_DB_PASS"
OMERO.server/bin/omero db script -f OMERO.server/db.sql --password "$OMERO_ROOT_PASS"
psql -h localhost -U "$OMERO_DB_USER" "$OMERO_DB_NAME" < OMERO.server/db.sql
To Deploy OMERO.web, you can use either Nginx or Apache. Follow the steps to install your chosen web server.
See also OMERO.web Nginx and Gunicorn deployment (Unix/Linux).
The following steps are run as root.
Install Nginx 1.8, install the requirements to run OMERO.web in the virtual environment, deactivate it and copy the Nginx OMERO configuration file into the Nginx configuration directory, and disable the default configuration:
cat << EOF > /etc/yum.repos.d/nginx.repo
[nginx]
name=nginx repo
baseurl=http://nginx.org/packages/centos/\$releasever/\$basearch/
gpgcheck=0
enabled=1
EOF
#install nginx
yum -y install nginx
virtualenv -p /usr/bin/python2.7 /home/omero/omeroenv
set +u
source /home/omero/omeroenv/bin/activate
set -u
# Install OMERO.web requirements
/home/omero/omeroenv/bin/pip2.7 install -r ~omero/OMERO.server/share/web/requirements-py27-nginx.txt
deactivate
# set up as the omero user.
su - omero -c "bash -eux setup_omero_nginx.sh"
mv /etc/nginx/conf.d/default.conf /etc/nginx/conf.d/default.disabled
cp ~omero/OMERO.server/nginx.conf.tmp /etc/nginx/conf.d/omero-web.conf
service nginx start
See also OMERO.web Apache and mod_wsgi deployment (Unix/Linux).
As the omero system user, configure OMERO.web by running the following commands:
OMERO.server/bin/omero config set omero.web.application_server wsgi
OMERO.server/bin/omero web config apache --http "$OMERO_WEB_PORT" > OMERO.server/apache.conf.tmp
OMERO.server/bin/omero web syncmedia
The following steps are run as root.
The version of Apache installed is 2.2, see the note below if you wish to use version 2.4.
Install Apache 2.2, install the requirements to run OMERO.web in the virtual environment, deactivate it and add the path to the virtual environment python to the python-path parameter of the WSGIDaemonProcess directive:
#install Apache 2.2
yum -y install httpd
# install mod_wsgi compiled against 2.7
yum -y install python27-mod_wsgi
virtualenv -p /usr/bin/python2.7 /home/omero/omeroenv
set +u
source /home/omero/omeroenv/bin/activate
set -u
# Install OMERO.web requirements
/home/omero/omeroenv/bin/pip2.7 install -r ~omero/OMERO.server/share/web/requirements-py27-apache.txt
deactivate
# Add virtual env python to the python-path parameter of the WSGIDaemonProcess directive
sed -i 's/\(python-path\=\)/\1\/home\/omero\/omeroenv\/lib64\/python2.7\/site-packages:/' ~omero/OMERO.server/apache.conf.tmp
cp ~omero/OMERO.server/apache.conf.tmp /etc/httpd/conf.d/omero-web.conf
chkconfig httpd on
service httpd start
Note
If you wish to use Apache 2.4 with mod_wsgi, see omero-install for a possible solution.
The following steps are run as the omero system user.
OMERO should now be set up. To start the server run:
OMERO.server/bin/omero admin start
If you deploy with Nginx, to start the OMERO.web client run:
OMERO.server/bin/omero web start
The last command is not necessary if you deploy with Apache.
Nginx or Apache should already be running so you should be able to log in as the OMERO root user by going to http://localhost/ in your web browser. Please read the SELinux section below.
In addition some example init.d scripts are available should you wish to start OMERO and OMERO.web automatically:
cp omero-init.d /etc/init.d/omero
chmod a+x /etc/init.d/omero
cp omero-web-init.d /etc/init.d/omero-web
chmod a+x /etc/init.d/omero-web
chkconfig --del omero
chkconfig --add omero
chkconfig --del omero-web
chkconfig --add omero-web
The following steps are run as root.
If multiple users have access to the machine running OMERO you should restrict access to OMERO.server’s configuration and runtime directories, and optionally the OMERO data directory:
chmod go-rwx ~omero/OMERO.server/etc ~omero/OMERO.server/var
# Optionally restrict access to the OMERO data directory
#chmod go-rwx "$OMERO_DATA_DIR"
The following steps are run as root.
The default OMERO.web session handler uses temporary files to store sessions which should be deleted at regular intervals, for instance by creating a cron job:
OMERO_USER=omero
OMERO_SERVER=/home/omero/OMERO.server
su - ${OMERO_USER} -c "${OMERO_SERVER}/bin/omero web clearsessions"
Copy the following commands into the appropriate location:
cp omero-web-cron /etc/cron.daily/omero-web
chmod a+x /etc/cron.daily/omero-web
The following steps are run as root.
If you are running a system with SELinux enabled (it is enabled by default on CentOS 6) and are unable to access OMERO.web you may need to adjust the security policy:
if [ $(getenforce) != Disabled ]; then
yum -y install policycoreutils-python
setsebool -P httpd_read_user_content 1
setsebool -P httpd_enable_homedirs 1
semanage port -a -t http_port_t -p tcp 4080
fi
The following steps are run as root.
It is possible to add Web applications to OMERO. If your app required some extra Python packages installed using pip, those packages should be also installed in the virtual environment. For example, OMERO.figure requires reportlab and markdown:
virtualenv -p /usr/bin/python2.7 /home/omero/omeroenv
source /home/omero/omeroenv/bin/activate
/home/omero/omeroenv/bin/pip2.7 install reportlab markdown