MATLAB is a high-level language and interactive environment that facilitates rapid development of algorithms for performing computationally intensive tasks.

Calling Bio-Formats from MATLAB is fairly straightforward, since MATLAB has built-in interoperability with Java. We have created a toolbox for reading and writing image files. Note the minimum recommended MATLAB version is R2017b.


It is possible to run Bio-Formats 6 on earlier MATLAB versions using a JVM version 8 or greater although using a different JVM than the one shipped with MATLAB can affect other functionalities. Please refer to the MATLAB Answers for more information.


Download the MATLAB toolbox from the Bio-Formats downloads page. Unzip and add the unzipped bfmatlab folder to your MATLAB path.


Please see Using Bio-Formats in MATLAB for usage instructions. If you intend to extend the existing .m files, please also see the developer page for more information on how to use Bio-Formats in general.


In our tests (MATLAB R14 vs. java 1.6.0_20), the script executes at approximately half the speed of our showinf command line tool, due to overhead from copying arrays.


If you encounter an error trying to open JPEG-2000 data in MATLAB but the file will open e.g. in Fiji using Bio-Formats, it may be due to conflicting versions of JAI ImageIO in different JARs. As discussed on the component page, JAI ImageIO is no longer maintained and you will likely need to remove the conflicting JAR(s) as a workaround.


To use a newer version of Bio-Formats, overwrite the content of the bfmatlab folder with the newer version of the toolbox and restart MATLAB.

Alternative scripts

Several other groups have developed their own MATLAB scripts that use Bio-Formats, including the following: