Micro-Manager

Micro-Manager is a software framework for implementing advanced and novel imaging procedures, extending functionality, customization and rapid development of specialized imaging applications.

Micro-Manager offers the functionality for saving the acquired images in TIFF/OME-TIFF format. Based on the mode of saving and the configuration settings, the acquired image can be saved with or without a companion file (*metadata.txt):

Saving options in Micro-Manager

Micro-Manager saving option

Micro-Manager saving format

Companion file

Bio-Formats reading

Reader users

Save as separate image files

TIFF

Yes

Full support

MicromanagerReader

Save as image stack file

OME-TIFF

No

Pixel data plus minimal metadata*

OMETiffReader

Save as image stack file

OME-TIFF

Yes**

Full Support

MicromanagerReader

* Not all acquisition metadata is converted to OME-XML.

** A small change in the acquisition side facilitates better handling of the metadata from the Bio-Formats side: Tools ‣ Options… and then select “Create metadata.txt file with Image Stack Files” in the text box.

See also

Micro-Manager User’s Guide - Files on Disk