Micro-Manager ============= `Micro-Manager `_ is a software framework for implementing advanced and novel imaging procedures, extending functionality, customization and rapid development of specialized imaging applications. Micro-Manager offers the functionality for saving the acquired images in TIFF/OME-TIFF format. Based on the mode of saving and the configuration settings, the acquired image can be saved with or without a companion file (:file:`*metadata.txt`): .. figure:: /images/micromanager_saving.png :width: 50% :align: center :alt: Saving options in Micro-Manager .. list-table:: :header-rows: 1 * - Micro-Manager saving option - Micro-Manager saving format - Companion file - Bio-Formats reading - Reader users * - Save as separate image files - TIFF - Yes - Full support - :doc:`/metadata/MicromanagerReader` * - Save as image stack file - OME-TIFF - No - Pixel data plus minimal metadata* - :doc:`/metadata/OMETiffReader` * - Save as image stack file - OME-TIFF - Yes** - Full Support - :doc:`/metadata/MicromanagerReader` ``*`` Not all acquisition metadata is converted to OME-XML. ``**`` A small change in the acquisition side facilitates better handling of the metadata from the Bio-Formats side: :menuselection:`Tools --> Options...` and then select "Create metadata.txt file with Image Stack Files" in the text box. .. seealso:: `Micro-Manager User's Guide - Files on Disk `_