Leica LAS AF LIF (Leica Image File Format)ΒΆ
Extensions: .lif
Developer: Leica Microsystems CMS GmbH
Owner: Leica
Support
BSD-licensed: 
Export:
Officially Supported Versions: 1.0, 2.0
Reader: LIFReader (Source Code, Supported Metadata Fields)
Freely Available Software:
- Leica LAS AF Lite (links at bottom of page)
We currently have:
- a LIF specification document (version 2, from no later than 2007 July 26, in PDF)
- a LIF specification document (version 1, from no later than 206 April 3, in PDF)
- numerous LIF datasets
We would like to have:
Ratings
Pixels: 
Metadata: 
Openness:
Presence: 
Utility:
Additional Information
Please note that while we have specification documents for this format, we are not able to distribute them to third parties.
LAS stands for “Leica Application Suite”. AF stands for “Advanced Fluorescence”.
Commercial applications that support LIF include:
Versions of Bio-Formats prior to 5.3.3 incorrectly calculated the physical pixel width and height. The physical image width and height were divided by the number of pixels, which was inconsistent with the official Leica LIF specification documents. Versions 5.3.3 and later correctly calculate physical pixel sizes by dividing the physical image size by the number of pixels minus one. To revert to the old method of physical pixel size calculation in 5.3.3 and later, set the leicalif.old_physical_size option to true. This can be done on the command line using showinf -option, in ImageJ via the configuration window, or via the API using the DynamicMetadataOptions class.